Confirmation of changes in the expression of several genes and representative findings of immunohistochemical staining in FLS and DS mice livers at 19 weeks of age. Real-time PCR analysis of liver tissue from mice (n = 18) revealed altered gene expression for (A) LCN2, (B) CXCL1, and (C) CXCL9, as detected by microarray analysis. Analysis results of the three genes were in directional concordance with microarray results and were statistically significantly upregulated in FLS compared with DS mice (p < 0.05). Differences between the FLS and DS groups were assessed by the Mann–Whitney U-test. (D) Hepatocytes strongly positive for LCN2 were aggregated close to most inflammatory foci, whereas DS mice hepatocytes were scarcely stained. LCN2 protein was also expressed in some mononuclear cells in both types of mice (arrowheads). (E) CXCL1 showed strong staining in some steatotic hepatocytes of FLS mice compared with faint staining in DS mice. (F) CXCL9 showed strong staining in the sinusoidal endothelium and hepatocytes present in the vicinity of some inflammatory cell clusters in FLS mice (asterisks), whereas DS mice hepatocytes were scarcely stained.