Induction of the renal accumulation of PPL near the tubules of obstructed kidneys. (A) Representative images for the localization of PPL in the cortex, outer medulla, and inner medulla of the contralateral (unobstructed) and obstructed kidneys. Results from wild-type mice subjected to unilateral ureteral obstruction for 7 days are shown. PPL was detected by red fluorescence. Scale bar: 100 μm. (B) Magnified images for PPL localization in the cortex and medulla of obstructed kidneys. The arrow indicates the urinary pole of the corpuscle. Scale bar: 25 μm. (C) Partial overlapping of the localization of PPL and aquaporin 2 (AQP2) in the cortex and outer medulla of contralateral (Cont.) and obstructed (Obst.) kidneys. PPL and AQP2 were double-stained by red and green fluorescence, respectively. Merged images are shown on the right. Scale bar: 100 μm. (D) Partial overlapping of the localization of PPL and K19 in the cortex and outer medulla of contralateral and obstructed kidneys. PPL and K19 were double-stained by red and green fluorescence, respectively. Scale Bar: 100 μm. (E) Immunohistochemical signals for PPL were absent in the obstructed renal cortex of Ppl
−/− mice. Scale bar: 100 μm. Asterisks in each panel indicate glomeruli.