Immunohistochemical localization of PPL in the cholestatic liver. (A) Representative images for the hepatic localization of PPL in mice fed the control or 1% CA-containing diet for 7 days. PPL was detected by red fluorescence. Scale bar: 100 μm. (B) PPL and ZO-1 (left column), or PPL and γ-CTN (right column), were double-stained by red and green fluorescence, respectively. Merged images are shown at the bottom. Arrowheads indicate PPL expression at ZO-1-negative sites (left bottom). Scale bar: 25 μm. (C) PPL and K19 were double-stained by red and green fluorescence, respectively. Signals for PPL were absent in cholangiocytes and hepatocytes of 1% CA-fed Ppl
−/− mice. Scale bar: 100 μm. (D) Protein abundance of the selected hepatic structural molecules in the liver of CA-fed mice at indicated doses for 7 days. Four mice per group were examined by western blotting analysis. The immunoblots for GAPDH were used as the loading controls. PV, portal vein; CV, central vein.