Table 1 RT-PCR Primer sequences
Cytokines | Primer sequence |
|---|---|
IFN-γ (90 bp) | Upstream 5′-GATGACTTCGAAAAGCTGACTAATTATTC-3′ |
Downstream 5′-GTTCAGCCATCACACTTGGATGAG-3′ | |
IL-12 (100 bp) | Upstream 5′-ATGATGGCCCTGTGCCTTAG-3′ |
Downstream 5′-TGCC TCTTAGGATCCATCCATCAGAAG-3′ | |
IL-4 (93 bp) | Upstream 5′-CACAAGCAGCTGATCCGATTC-3′ |
Downstream 5′-TCTGGTTGGCTTCC TTCACAG-3′ | |
IL-10 (123 bp) | Upstream 5′-CACAAGCAGCTGAT CCGATTC-3′ |
Downstream 5′-TCTGGTTGGCTTCCTTCACAG-3′ | |
β-actin (98 bp) | Upstream 5′-AGGTCATCACCATTGGCAATG-3′ |
Downstream 5′-GGTAGTTTCGTGGATGCCACA-3′ |