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Table 1 qPCR analysis for hepatic markers in differentiated UCMSCs

From: Systemic administration of a novel human umbilical cord mesenchymal stem cells population accelerates the resolution of acute liver injury

 

7 days

*p

14 days

*p

21 days

*p

28 days

*p

Â¥p

Petri dishes

AFP

0.481

0.0402

0.573

0.0377

0.358

0.0485

0.124

0.0003

0.0378

 

(0.472; 0.482)

 

(0.566; 0.575)

 

(0.357; 0.364)

 

(0.122; 0.124)

 

(z = −2.08)

Alb

2.652

0.0158

4.298

0.0100

5.991

0.0118

7.880

0.0076

0.0219

 

(2.630; 2.683)

 

(4.276; 4.438)

 

(5.966; 6.261)

 

(7.718; 8.144)

 

(z = 2.30)

MTP

1.012

NS

1.924

0.0051

3.606

0.0096

4.805

0.0007

0.0021

 

(1.007; 1.017)

 

(1.910; 1.929)

 

(3.521; 3.612)

 

(4.741; 4.821)

 

(z = 3.08)

TDO

7.464

0.0144

10.483

0.0098

16.111

0.0091

19.575

0.0011

0.0023

 

(7.380; 7.576)

 

(10.301; 10.601)

 

(16.040; 16.295)

 

(19.497; 19.802)

 

(z = 3.12)

Matrigel TM

AFP

0.719

0.0450

0.592

NS

0.638

0.0202

0.587

0.0091

NS

 

(0.715; 0.719)

 

(0.591; 0.596)

 

(0.635; 0.639)

 

(0.583; 0.589)

  

Alb

0.704

0.0405

1.437

NS

1.926

NS

2.486

0.0196

0.0065

 

(0.701; 0.704)

 

(1.419; 1.445)

 

(1.895; 1.945)

 

(2.446; 2.520)

 

(z = 2.72)

MTP

0.789

NS

0.814

NS

2.452

0.0092

2.274

0.0091

0.0122

 

(0.781; 0.790)

 

(0.813; 0.820)

 

(2.432; 2.461)

 

(2.258; 2.278)

 

(z = 2.51)

TDO

1.250

NS

1.210

NS

1.269

NS

1.490

NS

NS

 

(1.170; 1.317)

 

(1.140; 1.374)

 

(1.258; 1.428)

 

(1.443; 1.594)

  

hLAM

AFP

0.992

NS

0.823

NS

0.778

NS

0.871

NS

NS

 

(0.982; 0.994)

 

(0.819; 0.829)

 

(0.777; 0.788)

 

(0.837; 0.876)

  

Alb

1.580

0.0353

0.860

NS

0.834

NS

0.788

NS

NS

 

(1.570; 1.595)

 

(0.859; 0.867)

 

(0.822; 0.847)

 

(0.786; 0.793)

  

MTP

nd

 

nd

 

nd

 

nd

  

TDO

nd

 

nd

 

nd

 

nd

  
  1. *p Mann–Whitney U-test ¥p Cuzick’s trend test.
  2. Values represent median (Q1; Q3) of 5 independent experiments. Data were analyzed using the second-derivative algorithm. For each sample, the quantity of specific hepatic marker mRNA was expressed as n-fold the normalized amount of mRNA from undifferentiated control cells.
  3. The Mann–Whitney U-test was used for the statistical analysis of differences between undifferentiated control cells and differentiated cells at each time points (*p). Multiple comparisons to evaluate the changes of a single mRNA expression during the time were performed with the Cuzick’s trend test (¥p). nd: not detected; NS: not significant.