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Table 1 qPCR analysis for hepatic markers in differentiated UCMSCs

From: Systemic administration of a novel human umbilical cord mesenchymal stem cells population accelerates the resolution of acute liver injury

  7 days *p 14 days *p 21 days *p 28 days *p ¥p
Petri dishes
AFP 0.481 0.0402 0.573 0.0377 0.358 0.0485 0.124 0.0003 0.0378
  (0.472; 0.482)   (0.566; 0.575)   (0.357; 0.364)   (0.122; 0.124)   (z = −2.08)
Alb 2.652 0.0158 4.298 0.0100 5.991 0.0118 7.880 0.0076 0.0219
  (2.630; 2.683)   (4.276; 4.438)   (5.966; 6.261)   (7.718; 8.144)   (z = 2.30)
MTP 1.012 NS 1.924 0.0051 3.606 0.0096 4.805 0.0007 0.0021
  (1.007; 1.017)   (1.910; 1.929)   (3.521; 3.612)   (4.741; 4.821)   (z = 3.08)
TDO 7.464 0.0144 10.483 0.0098 16.111 0.0091 19.575 0.0011 0.0023
  (7.380; 7.576)   (10.301; 10.601)   (16.040; 16.295)   (19.497; 19.802)   (z = 3.12)
Matrigel TM
AFP 0.719 0.0450 0.592 NS 0.638 0.0202 0.587 0.0091 NS
  (0.715; 0.719)   (0.591; 0.596)   (0.635; 0.639)   (0.583; 0.589)   
Alb 0.704 0.0405 1.437 NS 1.926 NS 2.486 0.0196 0.0065
  (0.701; 0.704)   (1.419; 1.445)   (1.895; 1.945)   (2.446; 2.520)   (z = 2.72)
MTP 0.789 NS 0.814 NS 2.452 0.0092 2.274 0.0091 0.0122
  (0.781; 0.790)   (0.813; 0.820)   (2.432; 2.461)   (2.258; 2.278)   (z = 2.51)
TDO 1.250 NS 1.210 NS 1.269 NS 1.490 NS NS
  (1.170; 1.317)   (1.140; 1.374)   (1.258; 1.428)   (1.443; 1.594)   
hLAM
AFP 0.992 NS 0.823 NS 0.778 NS 0.871 NS NS
  (0.982; 0.994)   (0.819; 0.829)   (0.777; 0.788)   (0.837; 0.876)   
Alb 1.580 0.0353 0.860 NS 0.834 NS 0.788 NS NS
  (1.570; 1.595)   (0.859; 0.867)   (0.822; 0.847)   (0.786; 0.793)   
MTP nd   nd   nd   nd   
TDO nd   nd   nd   nd   
  1. *p Mann–Whitney U-test ¥p Cuzick’s trend test.
  2. Values represent median (Q1; Q3) of 5 independent experiments. Data were analyzed using the second-derivative algorithm. For each sample, the quantity of specific hepatic marker mRNA was expressed as n-fold the normalized amount of mRNA from undifferentiated control cells.
  3. The Mann–Whitney U-test was used for the statistical analysis of differences between undifferentiated control cells and differentiated cells at each time points (*p). Multiple comparisons to evaluate the changes of a single mRNA expression during the time were performed with the Cuzick’s trend test (¥p). nd: not detected; NS: not significant.