Table 1 List of primary (A) and secondary (B) antibodies used for Immunofluorescence (IF), Flow Cytometry (FC) and Western blot (WB) analysis
A. | ||||
|---|---|---|---|---|
Primary Ab | Host | Dilution | Specificity | Source |
Ki67 | Rabbit | 1:100 FC | Proliferating cells | Abcam |
Nestin | Mouse | 1:2000 WB, IF | Neural progenitor cells | BD |
βIII-Tubulin | Mouse | 1:2000 IF; 1:5000 WB | Neurons | Promega |
Chicken | 1:2000 IF | Neurons | Aves Laboratories | |
PGP 9.5 | Rabbit | 1:2000 WB, IF | Neurons | Chemicon |
Peripherin | Rabbit | 1:2000 WB, IF | Neurons | Chemicon |
MAP-2 | Chicken | 1:2000 WB, IF | Neurons | Aves Laboratories |
nNOS | Mouse | 1:2500 WB | Neurons | BD |
Rabbit | 1:200 IF | Neurons | Zymed | |
GFAP | Rabbit | 1:2000 IF 1:5000 WB | Glial cells | DAKO |
α-SMA | Rabbit | 1:1000 IF 1:5000 WB | Smooth muscle cells | Abcam |
GAPDH | Mouse | 1:10000 WB | Loading control | Abcam |
B. | ||||
Secondary Ab | Host | Dilution | Source | |
Alexa 633 anti rabbit | Goat | 1:200 FC | Molecular Probes | |
Alexa 594 anti mouse or rabbit | Goat | 1:200 IF | Molecular Probes | |
Alexa 488 anti mouse or rabbit | Goat | 1:200 IF | Molecular Probes | |
HRP conjugated anti mouse or Rabbit | Goat | 1:20000 WB | Zymed | |