Figure 7

Intrapyloric transplantation of ES-NS . (A) Grafted DiI-labeled ES-NS (red) after 7 days from transplantation were found mainly in the muscle and submucosal layers. (B-D) Immunofluorescence staining revealed a failure by the grafted ES-NS to express mature phenotype markers such as peripherin (green, B), GFAP (green, C) and α-smooth muscle actin (green, D). Nuclear staining with DAPI (blue). M, muscle; SM, submucosa; Mu, mucosa. Calibration bars = 25 μm.