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Figure 3 | BMC Gastroenterology

Figure 3

From: Evaluation of ES-derived neural progenitors as a potential source for cell replacement therapy in the gut

Figure 3

Time course analysis of small intestine longitudinal muscle – myenteric plexus (LM-MP) preparations. Immunoreactivity for the neuronal marker βIII-tubulin (red, A-B) and for the glial marker GFAP (red, C-D) reveal a preserved anatomy of the myenteric plexus and its ganglia (insets) in mouse small intestine LM-MP strips maintained in culture in NB27 medium for 5 days (B, D) compared to fresh preparations (A, C). Similarly, the persisting reactivity for α-smooth muscle actin, α-SMA (red, E-F) shows the longitudinal muscle still intact after 5 days in culture (F, fresh preparation control in E). Nuclei are counterstained with DAPI (blue). Calibration bars = 50 μm.

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