Comparative analysis of inflamed and non-inflamed colon biopsies reveals strong proteomic inflammation profile in patients with ulcerative colitis

Table 1 List of significant proteins showing more than twofold altered protein expression within UC patients

Spot	Protein Name	SwissProt Acc. no.	S cov %¹	pI²	Mw²	Fold diff.³	T -test (p)⁴
Higher level in RE
1	Glycerol-3-phosphate-dehydrogenase	P04406	6^a	9.3	36	3.30	1.388e-005
2⁵	B-cell antigen receptor complex-associated protein beta chain	P40259	42	5.6	26	3.05	3.776e-004
3	Annexin A6	P08133	41^a	5.3	76	2.81	1.626e-006
4	Glycerol-3-phosphate-dehydrogenase	P04406	37^a	9.3	36	2.70	6.178e-005
5	Plasma-cell induced resident endoplasmatic reticulum protein	Q8WU39	52^a	5.3	21	2.61	4.785e-005
6⁵	Actin cytoplasmic	P60709	42^a	5.5	41	2.45	1.049e-006
7	Alpha enolase	P06733	35^a	7.7	47	2.42	5,889e-005
8	L-lactate dehydrogenase B-chain	P07195	46^a	5.7	37	2.40	4.021e-005
9	Tubulin beta 5-chain	P07437	41^a	4.6	50	2.18	1.801e-004
Lower level in RE
10	Alpha-enolase	P06733	44^a	7.7	47	3.70	1.403e-008
11	Hydroxymethylglutaryl–CoA synthase	P54868	28^a	9.2	57	2.94	2.448e-009
12	Selenium binding protein	Q13228	42^a	5.9	53	2.50	3.770e-007
13	Carbonic anhydrase 2	P00918	60^a	7.0	29	2.33	8.325e-007
14	Selenium binding protein	Q13228	60^a	5.9	53	2.08	5.508e-006

¹Percentage sequence coverage (S cov %) is followed by ^a if the mascot score was significant (P < 0.05). ²Isoelectric point (pI) and molecular weight (Mw) are presented as theoretical values. ³Fold difference in protein level. ⁴p-value from t-test. ⁵Spot location on the gel differs from the theoretical molecular mass. ⁶The protein was identified by MS/MS.

ISSN: 1471-230X