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Figure 3 | BMC Gastroenterology

Figure 3

From: Hepatitis C virus E2 protein involve in insulin resistance through an impairment of Akt/PKB and GSK3β signaling in hepatocytes

Figure 3

HCV E2 induce SOCS3 mRNA and protein levels in Huh7. Huh7 cells were transfected with FLAG-CMV2 or FLAG-E2 for 24 hours and then subjected to semi-quantitative PCR for SOCS3 expression with GAPDH being an internal control (A). For western blotting, cell lysate samples were prepared from Huh7 cells transfected with FLAG-E2 for the indicated time and then subjected to western blotting for SOCS3 with β-actin and IL-6 treatment (100 ng/ml for 30 minutes) being an internal control and positive control, respectively. Results in (B) indicated that the expression levels of SOCS3 were increased by E2 expression.

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BMC Gastroenterology

ISSN: 1471-230X

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