Table 1 Primer sequences, probes and accession numbers of transcripts, used for RT PCR quantification
From: Mitochondrial uncouplers inhibit hepatic stellate cell activation
Gene | Primers sequence (Left primer/Right primer) | Probe | GenBank accession number | Product lenght |
|---|---|---|---|---|
18S | 5′-aaatcagttatggttcctttggtc-3′/ | 55 | AY_248756 | 67 |
5′gctctagaattaccacagttatccaa-3′ | ||||
PGC1α | 5′- cagtcgcaacatgctcaag-3′/ | 6 | NM_008904 | 73 |
5′- tggggtcatttggtgactct-3′ | ||||
Tfam | 5′- caaaggatgattcggctcag-3′/ | 97 | NM_009360 | 92 |
5′- aagctgaatatatgcctgcttttc-3′ | ||||
CoxIV | 5′- tcactgcgctcgttctgat-3′/ | 7 | NM_37829.1 | 67 |
5′- cgatcgaaagtatgagggatg-3′ | ||||
Ndufs7 | 5′- gtggtgaccaagctggatg-3′/ | 104 | NM_029272 | 67 |
5′- cgaaggtcataggccacag-3′ | ||||
HO-1 | 5′- gtcaagcacagggtgacaga-3′/ | 4 | NM_010442 | 77 |
5′- atcacctgcagctcctcaaa-3′ | ||||
Smad6 | 5′- gttgcaacccctaccacttc -3′/ | 70 | NM_008542 | 76 |
5′- ggaggagacagccgagaata -3′ | ||||
Smad7 | 5′- acccccatcaccttagtcg -3′/ | 63 | NM_001042660 | 75 |
5′- gaaaatccattgggtatctgga -3′ | ||||
α-SMA | 5′-ccagcaccatgaagatcaag-3′/ | 58 | NM_007392 | 70 |
5′-tggaaggtagacagcgaagc-3′ | ||||
Pdgfrβ | 5′-tgcagagacctcaaaaggtg-3′/ | 63 | NM_008809.1 | 112 |
5′- cctgatcttcctcccagaaa-3′ | ||||
Procol1a1 | 5′-acctaagggtaccgctgga-3′/ | 19 | NM_007742 | 97 |
5′-tccagcttctccatctttgc-3′ | ||||
α-SMA* | 5′-ctgttccagccatccttcat-3′/ | NM_001141945 | 70 | |
5′-tcatgatgctgttgtaggtgg-3′ | ||||
PROCOL1A1* | 5′-gacacagaggtttcagtgg-3′/ | NM_000088.3 | 264 | |
5′-cacccttagcaccaacag-3′ | ||||
18S* | 5′-aagacggaccagagcgaaag-3′/ | K03432 | 98 | |
5′-tcggaactacgacggtatct-3′ | ||||
PDGFR-β* | 5′-cccttatcatcctcatcatgc-3′/ | NM_002609.3 | 60 | |
5′-ccttccatcggatctcgtaa-3′ |