Figure 3From: Mitochondrial uncouplers inhibit hepatic stellate cell activation Chemical uncoupling does not induce cytotoxicity on concentrations that inhibit HSC in vitro activation. (A) Cytotoxic effect of mitochondrial uncouplers, as measured by fluorescence of bis-alanylalanyl-phenylalanyl-rhodamine 110, a peptide substrate of cytoplasmic peptidases. Insert shows cells treated with cycloheximide as positive control (B) Caspase 3/7 activity after treatment of cells with different concentrations of Valinomycin and FCCP on different time points. Enzyme activity was measured as described in material and methods. * indicates P < 0.05 compared to control group.Back to article page