Figure 2
Effect of 1,25(OH) 2 D 3 on the expressions of TNF-α and IFN-γ. (A and B) Expressions of TNF-α and IFN-γ proteins were analyzed by immunohistochemistry in colon tissue from control group (a), model group (b) and 1,25(OH)2D3-treated group (c) (Immunohistochemistry staining; original magnifications, ×200). (C and D) Expressions of TNF-α and IFN-γ proteins in colon tissue were analyzed by Western blot. (E) Expressions of TNF-α mRNA and IFN-γ mRNA in colon tissue were analyzed by Real time Q-PCR. Data were expressed as mean ± SD. a P < 0.01 vs model group; b P < 0.01 vs control group.
