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Figure 1 | BMC Gastroenterology

Figure 1

From: The balance between two isoforms of LEF-1 regulates colon carcinoma growth

Figure 1

LEF-1 expression in colon cancer lines and primary tissue. (A) The expression of LEF-1 in primary tissue was examined by western blot. Every sample from the 22 patients was studied, and two representative samples are shown. 1a and 2a denote colon carcinomas; 1b and 2b denote adjacent tissues; 1c and 2c denote normal colon tissues. The molecular weight of full-length LEF-1 is about 55 KD and for LEF-1-ΔL it is 40 KD. (B) The expression of LEF-1 in different cell lines was examined by western blot. Control denotes Jurkat cell lysis (Invitrogen). (C) The expression of His tag (about 28 KD) and LEF-1 in SW480 and HT29 cells expressing LEF-1-ΔL or LEF-1-FL was examined by western blot. β-Actin was detected as a reference control. SW480-P is short for SW480-pCDNA3.1-His, and HT29-P is short for HT29- pCDNA3.1-His.

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