Figure 2

Effect of treatment with anti-HMGB1 antibody or sham IgG on serum ALT/AST in acetaminophen (APAP)-induced acute liver injury (ALI). ALI was induced in C57 BL/6 male mice with a single dose of APAP (350 mg/kg) by i.p. injection. 2 hours after APAP administration, the first dose of 400 μg of anti-HMGB1 antibody in 0.5 mL saline was i.p. injected into mice in the anti-HMGB1 group, the same amount of sham IgG or saline solution was given to the sham IgG and the control animals at the equivalent time points. The same dose was repeated 24 hrs after the first dose. Figure 2A, 2B: Serum ALT/AST was assessed 24 hrs after APAP injection from the anti-HMGB1 group, the sham IgG and the control group (n = 6 for each group). Figure 2C and 2D: 3 separate groups of mice were used. Serum ALT and AST were assessed at 48 h time point from the anti-HMGB1 group (n = 10), the sham IgG group (n = 9) and the control group (n = 6). Results are means ± SEM. * indicates p < 0.05 vs. control; † indicates p < 0.05 vs. sham IgG.