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Figure 8 | BMC Gastroenterology

Figure 8

From: Apolipoprotein E COG 133 mimetic peptide improves 5-fluorouracil-induced intestinal mucositis

Figure 8

A. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) analysis of apoptosis in IEC-6 cells were analyzed under 5% fetal bovine serum and exposed to 5-FU, with either ApoE (COG 133) or ApoA-I (D-4F) peptide treatment. Cells were stained using DeadEnd Fluorometric Tunel kit (head arrows). The Tunel System measures the fragmented DNA of apoptotic cells by catalytically incorporating fluorescein-12-dUTP at 3′-OH DNA ends using the enzyme TdT (Terminal deoxynucleotidyl Transferase). Data are plotted as mean TUNEL-labeled cells under high magnification (X400). Values represent mean ± SEM and were analyzed by one-way ANOVA. B. Cell necrosis and apoptosis were analyzed with flow cytometry in 1% fetal bovine serum and exposed to 5-FU with ApoE COG 133 peptide treatment. Cells were stained with FITC-conjugated annexin V and propidium iodide and analyzed by flow Calibur dual laser fluorescence-activated cell sorter (FACS). Results are shown as density plots with propidium iodide vs. annexin V-FITC. Bars on the graph represent the percentage of apoptotic cells have apoptotic and necrotic cells.

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