Figure 6

Dose response of the apoE COG 133 mimetic peptide (0.02-20 μM) on IEC-6 cell proliferation 24 h after 5-FU challenge (5-FU media exposure for 1 h) in glutamine-free media (A) or standard media (B), detected by formazan absorbance using an ELISA microplate reader at 450 nm in 96-well seeded IEC-6 cells. Values represent mean ± SEM and were analyzed by one-way ANOVA. (C) Ki67 immunolabeling of IEC-6 cells from chamber slides 24 h after 5-FU challenge (5-FU media exposure for 1 h) in glutamine-free media with either ApoE (COG 133) or ApoA-I (D-4F) peptides. Squares show areas of nested cells, suggesting a cloning cluster. Values represent mean% of Ki67-labeled cells in high magnified fields (X400) and were analyzed by one-way ANOVA.