Figure 3

Dectin-1 involvement in H. hepaticus induced colitis. Mice were inoculated intragastrically with H. hepaticus and received weekly i.p. injections of 1 mg anti-IL-10R for the duration of four weeks. A) Bodyweights were measured, weights are shown as percentage of weight on day 28 compared to day one. Disease severity was measured using B) spleen weight F) colon total inflammation score and G) cecum total inflammation score. C) Representative picture of healthy cecum. D) Representative picture of WT cecum after H. hepaticus induced colitis. E) Representative picture of dectin-1-/- cecum after H. hepaticus induced colitis. In inflamed cecum a large amount of cell infiltration was found in both the mucosa (arrow) and submucosa (asterisk). Bar, 500 um. H) IL-10 protein concentrations in colon lysates I) MCP-1 protein concentrations in colon lysates. The sample means are indicated with a line. A t-test was used for statistical analysis. *, p < 0.05, n = 5.