Functional capacity of ABCG2. A. The uptake of intracellular Hoechst 33342 accumulation measured by spectrofluorometry. The difference in intracellular dye accumulation with and without ABCB1 inhibitor verapamil was observed to be lower in JHH-6 followed by HepG2 and HuH-7 indicating major functional activity of ABCG2 in JHH-6. Cells without verapamil = 100.0%. B. Representative histogram plots showing the intracellular fluorescence of Rho123 in 10,000 events by FACS analysis. The data represented the mean ± SD of minimum three independent experiments. C. The dose-dependent intracellular Hoechst 33342 accumulation in monolayer HuH-7 and JHH-6 cells. Statistical student’s t test: *p<0.05.