Figure 4

Pantoprazole attenuated indomethacin-induced inflammatory mediators through HO-1. (A) RT-PCR for TNF-α, IL-1β, IL-8, and HO-1. 500 μM indomethacin increased the expression of inflammatory mediators including TNF-α, IL-1β, IL-8, but significantly decreased HO-1 expression. NOX-1 was significantly increased with indomethacin. These figures are representatives of the results obtained in 3 independent experiments. (B) Pantoprazole significantly decreased indomethacin-induced IL-1β and TNF-α, but these beneficiary actions of pantoprazole were abolished with ZnPPIX, leading to the conclusion that anti-inflammatory actions of pantoprazole were owing to HO-1 induction. These figures are representatives of the results obtained in 3 independent experiments. (C) Pantoprazole significantly decreased indomethacin-induced ICAM-1 and VCAM in HUVEC cells, but these beneficiary actions of pantoprazole were also abolished with ZnPPIX as HO-1 inhibitor. These figures are representatives of the results obtained in 3 independent experiments.