Figure 2
Pantoprazole increased Nrf2 nuclear translocation and cytoplasmic keap1 inactivation, led to significant anti-oxidation. (A) Western blots for either cytosolic Keap1 or nuclear Nrf2 in a different time in the presence of 300 μM pantoprazole. (B) Electrophoretic mobility shift assay (EMSA) for ARE-DNA binding activity. Significantly increased DNA-binding of Nrf2 was noted 60 min after pantoprazole administration. (C) Confocal imaging of Nrf2 after different dosing of pantoprazole, 100 μM and 300 μM. (D) Electron spin resonance (ESR) measurement for Fenton reaction-generated hydroxyl radicals. (E) The changes of DCF-DA fluorescence after different dosing of pantoprazole, 30, 100, and 300 μM. Pantoprazole decreased significantly H2O2-induced oxidative fluorescence.