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Table 4 Expression of tight junction-related components in esophageal mucosa in patients with GERD

From: Role of tight junction proteins in gastroesophageal reflux disease

Panel A
Transcript level
Gene expression/ß-actin (a.u.) median (range) Changevs.
controls (x-fold)
P-values (* Kruskal-Wallis; posthoc: Man WhitneyUtest)
Occludin    0.098*
controls 0.041 (0.0086 - 0.38)   
NERD 0.060 (0.0052 - 0.57) 1.46 n.a.
ERD 0.035 (0.0026 - 1.09) 0.85 n.a.
Claudin-1    0.0097*
controls 0.078 (0.0072 - 2.54)   
NERD 0.15 (0.012 - 1.6) 1.92 0.016
ERD 0.20 (0–4.1) 2.56 0.0032
Claudin-2    0.0027*
controls 0.000038 (0–0.003)   
NERD 0.0002 (0–0.019) 5.26 0.0041
ERD 0.000083 (0–0.021) 2.18 0.11
ZO-1    0.069*
controls 0.0060 (0.0012 - 0.073)   
NERD 0.0081 (0–0.067) 1.35 n.a.
ERD 0.0077 (0.0015 - 0.21) 1.28 n.a.
ZO-2    0.061*
controls 0.011 (0.0022 - 0.038)   
NERD 0.019 (0.002 - 0.27) 1.72 n.a.
ERD 0.021 (0.0019 - 0.59) 1.9 n.a.
Panel B
Protein level
IHC score
median (range)
Change (x-fold)
vs. controls
P -values (* Kruskal-Wallis;
posthoc: Man Whitney U test)
Occludin    0.02*
controls 3 (1 – 9)   -
NERD 6 (1 – 15) 2.0 0.026
ERD 8 (1 – 12) 2.7 0.012
Claudin-1    0.014*
controls 1 (1 – 4)   -
NERD 2 (1 – 9) 2.0 0.14
ERD 6 (1 – 8) 6.0 0.0004
Claudin-2    0.0057*
controls 4 (1 – 9)   -
NERD 6 (1 – 12) 1.5 0.28
ERD 9 (4 –15) 2.25 0.0025
ZO-1    0.62*
controls 2 (0 – 6)   -
NERD 4 (1 –12) 2.0 n.a.
ERD 3 (0 – 3) 1.5 n.a.
ZO-2    0.58*
controls 4 (0 – 10)   -
NERD 4 (0 – 8) 1.0 n.a.
ERD 1 (0 – 8) 0.25 n.a.
  1. Transcript levels are shown in relation to controls (Panel A). Immunohistochemical scores are illustrated similarly (Panel B). Statistical analyses for both datasets were done first by non-parametric Kruskal-Wallis (P-value italic style); if significant post-hoc analysis was done by Mann Whitney U test. Significant changes are demonstrated by bold letters. n.a.: not applicable.