Table 4 Expression of tight junction-related components in esophageal mucosa in patients with GERD

Occludin

0.098*

controls

0.041 (0.0086 - 0.38)

NERD

0.060 (0.0052 - 0.57)

1.46

n.a.

ERD

0.035 (0.0026 - 1.09)

0.85

n.a.

Claudin-1

0.0097*

controls

0.078 (0.0072 - 2.54)

NERD

0.15 (0.012 - 1.6)

1.92

0.016

ERD

0.20 (0–4.1)

2.56

0.0032

Claudin-2

0.0027*

controls

0.000038 (0–0.003)

NERD

0.0002 (0–0.019)

5.26

0.0041

ERD

0.000083 (0–0.021)

2.18

0.11

ZO-1

0.069*

controls

0.0060 (0.0012 - 0.073)

NERD

0.0081 (0–0.067)

1.35

n.a.

ERD

0.0077 (0.0015 - 0.21)

1.28

n.a.

ZO-2

0.061*

controls

0.011 (0.0022 - 0.038)

NERD

0.019 (0.002 - 0.27)

1.72

n.a.

ERD

0.021 (0.0019 - 0.59)

1.9

n.a.

Panel B

Protein level

IHC score

median (range)

Change (x-fold)

vs. controls

P -values (* Kruskal-Wallis;

posthoc: Man Whitney U test)

Occludin

0.02*

controls

3 (1 – 9)

-

NERD

6 (1 – 15)

2.0

0.026

ERD

8 (1 – 12)

2.7

0.012

Claudin-1

0.014*

controls

1 (1 – 4)

-

NERD

2 (1 – 9)

2.0

0.14

ERD

6 (1 – 8)

6.0

0.0004

Claudin-2

0.0057*

controls

4 (1 – 9)

-

NERD

6 (1 – 12)

1.5

0.28

ERD

9 (4 –15)

2.25

0.0025

ZO-1

0.62*

controls

2 (0 – 6)

-

NERD

4 (1 –12)

2.0

n.a.

ERD

3 (0 – 3)

1.5

n.a.

ZO-2

0.58*

controls

4 (0 – 10)

-

NERD

4 (0 – 8)

1.0

n.a.

ERD

1 (0 – 8)

0.25

n.a.