Skip to main content

Table 1 PCR conditions for genotypes of test DNA genes in the study

From: Multi-susceptibility genes associated with the risk of the development stages of esophageal squamous cell cancer in Feicheng County

Gene Primer sequence Annealing Restriction enzyme
MTHFR
C677T
F: 5'-TGA AGG AGA AGG TGT CTG CGG GA-3'
R: 5'-AGG ACG GTG CGG TGA GAG TG-3'
62°C Hinf I
CYP2E1
G1259C
F: 5'-CCA GTC GAG TCT ACA TTG TCA-3'
R: 5'-TTC ATT CTG TCT TCT AAC TGG-3'
55°C PstI
hOGG1
C326G
F: 5'-GGA AGG TGC TTG GGG AAT-3'
R: 5'-ACT GTC ACT AGT CTC ACC AG-3'
58°C Fnu4HI
MPO
G463A
F: 5'-CGG TAT AGG CAC ACA ATG GTG AG-3'
R: 5'-GCA ATG GTT CAA GCG ATT C-3'
62°C AciI
ALDH2 F1: 5'-TCA TGC CAT GGC AAC TCC AGC-3'
R1: 5'-CCC ACA CTC ACA GTT TTC TCT TC-3'
F2: 5'-TAC GGG CTG CAG GCA TAC ACT A-3
R2: 5'-TGA TCC CCA GCA GGT CCT GAA-3'
60°C