Table 1 PCR conditions for genotypes of test DNA genes in the study

MTHFR

C677T

F: 5'-TGA AGG AGA AGG TGT CTG CGG GA-3'

R: 5'-AGG ACG GTG CGG TGA GAG TG-3'

62°C

Hinf I

CYP2E1

G1259C

F: 5'-CCA GTC GAG TCT ACA TTG TCA-3'

R: 5'-TTC ATT CTG TCT TCT AAC TGG-3'

55°C

PstI

hOGG1

C326G

F: 5'-GGA AGG TGC TTG GGG AAT-3'

R: 5'-ACT GTC ACT AGT CTC ACC AG-3'

58°C

Fnu4HI

MPO

G463A

F: 5'-CGG TAT AGG CAC ACA ATG GTG AG-3'

R: 5'-GCA ATG GTT CAA GCG ATT C-3'

62°C

AciI

ALDH2

F1: 5'-TCA TGC CAT GGC AAC TCC AGC-3'

R1: 5'-CCC ACA CTC ACA GTT TTC TCT TC-3'

F2: 5'-TAC GGG CTG CAG GCA TAC ACT A-3

R2: 5'-TGA TCC CCA GCA GGT CCT GAA-3'

60°C