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Figure 4 | BMC Gastroenterology

Figure 4

From: Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines

Figure 4

Sphere-forming cells overexpressed liver CSC related proteins and downstream proteins of the CSL-independent Notch pathway. (A) The PLC/PRF/5 sphere-forming cells expressed the hepatic stem cell maker OV6, as observed under fluorescence microscopy. Nuclei were stained with DAPI (400×). (B) Western blotting assay showed the liver CSC related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44) and the relative band intensities were calculated by densitometry and normalized to the loading control GAPDH.

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