Sample | Group | IgA+ cells | Goblet cells | CD4+ TL | CD8+ TL |
|---|
Basal | WC | 191 ± 36 a
| 46 ± 14 a
| 43 ± 11 a
| 30 ± 9 a,b
|
| | MC | 147 ± 44 a,b
| 42 ± 23 a
| 23 ± 8 b
| 17 ± 6 a
|
5 days of re-nutrition | WC | 143 ± 26 a,b
| 51 ± 13 a
| 47 ± 9 a
| 40 ± 7 b
|
| | MC | 106 ± 33 b
| 41 ± 14 a
| 30 ± 7 b,c
| 24 ± 7 a
|
| | Milk | 100 ± 32 b
| 48 ± 27 a
| 53 ± 17 a,c
| 26 ± 10 a,b
|
| | PFM | 163 ± 31 a,b
| 34 ± 9 a
| 36 ± 9 a,b
| 20 ± 5 a
|
| | BFS | 124 ± 39 a,b
| 32 ± 8 a
| 28 ± 8 a,b
| 24 ± 8 a
|
- The number of IgA+ cells, CD4+ and CD8+ T lymphocytes were determined by direct immunofluorescence on the small intestine tissue slides of mice from different experimental groups (well-nourished control (WC), malnourished control (MC) and re-nourished with milk, probiotic fermented milk (PFM) or bacterial free supernatant (BFS)). The results were expressed as the number of positive cells per ten fields of vision (1000X). Goblet cells were stained with alcian blue on slides from the small intestine of mice from different groups under study and the results were expressed as number of positive cells per 10 villi. Values are means for n = 9 ± SD mice from each group and sample. a,b,c Means for each cell population without a common letter differ significantly (P < 0.05).
