Figure 5

Slug silencing inhibits seeded tumor in a pseudometastatic model of ESCCin vivo.. Seeded tumor in abdominal cavity organ(A-F)and Seeded tumor in liver (G-I) of each animal was evaluated for the extent of metastatic invasion. Seeded tumor in abdominal cavity was showed in parental (A)and siRNA mock-transfected mice(B), fewer seeded tumors in abdominal cavity were seen in siRNA Slug transfected mice(C). Seeded tumor in abdominal cavity organ was showed in parental (D)and mock transfected mice(E), fewer seeded tumors in abdominal cavity organ were seen in siRNA Slug transfected mice(F). Seeded tumor in liver was showed in control(G)and mock transfected mice(H), fewer seeded tumors in liver were seen in siRNA Slug transfected mice(I). Significant seeded tumor differences between the control and mock transfected groups are indicated(P < 0.05) (J),( magnification ×200).