Slug affects proliferation in ESCC cells. A,B,MTT assay was done to investigate proliferation of siRNA-transfected EC109 cells and Slug cDNA-transfected TE13 cells. Every 24 h, the absorbencies of test well were read and semilogarithmic curves were drawn. Points, mean of three separate experiments; bars, SD. C,D, Down regulation of Slug inhibited colony forming ability, and upregulation of Slug promotes colonyforming ability. Histogram was the average number of colonies in each group.