Slug regulates ESCC cells invasion in vitro. A,B, Stable Slug-transfected EC109 cells or Slug cDNA-transfected TE13 cells(1.25 × 105) were seeded into the upper chamber of the Transwell and the lower chamber was filled with 0.8 mL NIH-3T3 conditioned medium to induce chemotaxis. After 24 h of incubation at 37°C, the cells that invasion through the pores to the lower surface of the filter were counted under a microscope. Three invasion chambers were used per condition. The values obtained were calculated by averaging the total number of cells from three filters.C,D, Slug Protein expression was determined by Western blot in stable Slug-transfected EC109 cells or Slug cDNA-transfected TE13 cells.