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Figure 1 | BMC Gastroenterology

Figure 1

From: Isoflurane Preconditioning at Clinically Relevant Doses Induce Protective Effects of Heme Oxygenase-1 on Hepatic Ischemia Reperfusion in Rats

Figure 1

Effect of isoflurane preconditioning on liver heme oxygenase-1(HO-1) after ischemia reperfusion injury (IR) (n = 12 each group). (A) The HO-1 activity in the liver. Both isoflurane and hemin pretreatment augmented the HO-1 activity response, while administration of the HO-1 inhibitor zinc protoporphyrin (Znpp), prior to the isoflurane pretreatment or Znpp alone significantly decreased the HO-1 activity (p < 0.05, vs. IR). (B) Western blot analysis of HO-1in liver. Western blotting showed an increase in HO-1 protein expression in the groups of isoflurane and hemin (* P < 0.05 vs. IR). (C) Immunohistochemical detection of HO-1 in the liver. Hepatic HO-1 positive cells were defined as stained with brown in cytoplasm (black arrows). There was an increase in HO-1 expression in isoflurane and hemin pretreated groups compared in IR, it also showed a decrease in HO-1protein expression in both Znpp pretreated groups compared with isoflurane group (Magnification: 400×. Scale bar = 100 μm).

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