Figure 3

Effects of LPS or TNF-α on release of cytokines from HepG2 cells. HepG2 cells were stimulated with LPS (200 ng/mL) or TNF-α (25 ng/mL) for 24 h, and extracellular levels of 42 different cytokines were determined by using human cytokine antibody array. A, B). Representative cytokine antibody array. Note that TNF, but not LPS, markedly induced the release of IL-8 and GRO. C). Quantitation of relative levels of IL-8 and GRO. The relative levels of IL-8 and GRO were determined using the UN-SCAN-IT Gel 6.1 software, and the optical density (OD) was expressed as mean ± SEM of two different experiment. * P < 0.05 versus control group.