5-aminosalicylate reduces fascin expression and cell motility in colorectal epithelial cells, whereas sodium butyrate has a converse effect. A: HT29 cells were treated with a range of doses of either 5-ASA or butyrate as indicated. Attached cell yields and percentage of apoptotic cells were determined and these data represent the mean of three independent experiments performed in triplicate ±SEM. Also shown are the subsequent western blot analyses of fascin expression. These blots are representative of three independent experiments and were re-probed for α-tubulin to show even sample loading. SW480 lysate was included as a positive control having been shown previously to express relatively high levels of fascin [Qualtrough et al., 2009]. B: The effect of 10 mM 5-ASA or 1 mM butyrate treatment on cell motility was determined on HT29 cells as measured by Boyden chamber assay using 5%FBS as an attractant (**, p = <0.01; ***, p = <0.001). Three independent experiments were carried out in triplicate and the data are expressed as the mean ± S.E.M.