Effect of treatment with RLS or saline on PCNA in mice with ALI. PCNA staining was assessed 24 h, 48 h after induction of ALI (or sham procedure). Figure 7A, B and C, the method and treatment were the same as described in Figure 1A and 1B (n = 5 for each group). Figure 7D and 7E: three separate groups of mice were used. ALI was induced the same as Figure 1 A and B. 2 hrs after APAP challenge, the animals were given the same treatment every 12 hrs for a total of 48 hrs. PCNA was detected 48 hrs after APAP injection (n = 5 for each group). Results are means ± SEM. * indicates p < 0.05. Typical picture is shown.