Figure 2

Result of PXR immunohistochemical staining on esophageal biopsy specimens. (A) Esophagus of healthy controls is lined by a stratified squamous epithelium and is negative for PXR (200×). (B) The esophageal mucosa of patients with reflux esophagitis is damaged and inflamed and demonstrates a weak signal for PXR (brown) in the cytoplasm of epithelium (200×). (C) Barrett's epithelium is characterized by a single layer of columnar epithelium with the presence of typical goblet cells. Cytoplasmic as well as some nuclear PXR expression is observed (400×). (D) Cells of adenoma tissue show high levels of nuclear PXR expression (400×). (E) Quantification showed that the percentage of cases with more than 2% PXR-positive nuclei was significantly higher in EAC than in BE (P < 0.01).