Figure 7From: Evaluation of HIV protease and nucleoside reverse transcriptase inhibitors on proliferation, necrosis, apoptosis in intestinal epithelial cells and electrolyte and water transport and epithelial barrier function in mice Rates of apoptosis and necrosis of control group (A), of 70 ug/mL of nelfinavir (B), 100 ug/mL of indinavir (C), zidovudine (D) and didanosine (E) on intestinal epithelial IEC-6 cell apoptosis and necrosis after 24 h of exposure. Cells were stained with FITC-conjugated annexin V and propidium iodide and analyzed by flow cytometry. Results are shown as density plots with propidium iodide vs. annexin V-FITC. Viable cells have low annexin-V-FITC and low propidium iodide staining (lower-left quadrant), apoptotic cells have high annexin V-FITC and low propidium iodide staining (lower-right quadrant), and necrotic cells have high propidium iodide and annexin V-FITC staining (upper-right quadrant).Back to article page