Figure 6

Histological changes of HCC after short-term and low-dose epirubicin chemotherapy, control (n = 6) vs. epirubicin (n = 6). (a) Fluorescein-mediated TUNEL assay was performed to identify apoptotic nuclei (green), * P = 0.006. (b) Immunohistochemical staining of HCC by Ki-67 (brown) was done for examining the effect of epirubicin on the cell proliferation of HCC, * P < 0.0063. (c) Effect of short-term and low-dose epirubicin chemotherapy on tumor angiogenesis, arrows stand for the PECAM-1-positive blood vessels (* P = 0.0282).