Table 1 PCR-RFLP conditions for NOS2 gene polymorphisms and gene IL-1β.
Genes | Primers | Enzyme Time/T° | Fragments | Reference |
|---|---|---|---|---|
NOS2 Ser608Leu rs2297518 | F: 5' - TGTAAACCAACTTCCGTGGTG - 3' R: 5' - GTCTCTGCGGGTCTGAGAAG - 3' | Tsp509I (10 U/μL) 16 hs/65°C | 288 bp CC:113, 175 bp CT:113, 142 and 175 bp TT:113, 142 bp | 17 |
NOS2 -954G/C | F: 5' - CATATGTATGGGAATACTGTATTTCAG - 3' R: 5' - TCTGAACTAGTCACTTGAGG - 3' | BsaI (5 U/μL) 1 h/37°C | 573 bp GG:437, 136 bp GC: 573, 437 and 136 bp CC: 573 bp | 18 |
NOS2 -1173C/T | F:5' - CAAAGATCCTTGAGCTCTGA - 3' R:5' - CAACTACATTAGGGAGAAGTTGAG - 3' | BccI (10 U/μL) 1 h/37°C | 199 bp CC: 199 bp CT: 199, 132 bp TT: 132 bp | 33 |
IL-1β | F:5' - CATGTGACCTGCTCGTCAGT - 3' R:5' - CCCTAGGGATTGAGTCCACA - 3' | BccI (10 U/μL) 1 h/37°C | 372 bp 141 and 231 bp | - |