Figure 1

List of vectors constructed. UGT1A1 regulatory regions (c.-4076 to c.-1, Nos. 1 - 8), with differing combinations of polymorphic variations, were inserted into the luciferase reporter plasmid PGV-B2. The wild-type vector is No.1, and No.2 is a typical vector with regulatory regions showing Gilbert syndrome. The open box is a wild-type region between gtPBREM and the TATA box, including ten linked polymorphisms: c.-3152G>A, c.-2951A>G, c.-2743T>C, c.-2737T>C, c.-2726G>A, c.-2724AT[8], c.-2473T>G, c.-1352A>C, c.-689A>C and c.-364C>T. The gray box is a mutant-type region having mutant-type sequence corresponding to the wild-type region. Luc is luciferase cDNA. The name of the expression vector corresponds to the polymorphisms included and the type of vector. In the first part of the name, T and G respectively denote c.-3275T and c.-3275G. In the second part of the name, w and m refer to wild type and mutant-type region between gtPBREM and the TATA box. In the third part, (TA)6 and (TA)7 denote A(TA)6TAA and A(TA)7TAA. In the fourth part, wild, Gilbert and combination indicate that each vector has wild-type sequence, or typical mutant-type sequence in subjects suffering from Gilbert syndrome, or an artificial sequence constructed for this study.