Expression of TLR-4 associated signaling molecules. For assessment of TLR-4 and CD14 expression, livers were collected from male C57BL/6 and TLR-2-/- mice fed CD or MCDD for 8 weeks. Pre-developed assays for real-time PCR were used according to the manufacturer's instructions. Expression of each target mRNA was calculated relative to average values in the control group using a comparative CT method and presented as mean ± SEM of at least 4 observations/group. Statistical comparisons were made using two-way ANOVA and Tukey multiple comparisons test. ap < 0.05 compared to strain-matched mice fed control diet (CD); bp < 0.05 compared to diet-matched wild type (WT) mice; cp < 0.05 compared to strain-matched mice fed PUFA.