Hepatic TNF-α and Collagen mRNA levels. Expression of TNF-α and Collagen α1 mRNA was analyzed by real-time PCR and calculated relative to average values of mice fed control diet (CD) using a comparative CT method. Data are presented as mean ± SEM of 4 observations/group. Statistical comparisons were made using one-way ANOVA. ap < 0.05 compared to mice fed CD; bp < 0.05 compared to mice fed PUFA.