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Figure 5 | BMC Gastroenterology

Figure 5

From: Effective in vivo and ex vivogene transfer to intestinal mucosa by VSV-G-pseudotyped lentiviral vectors

Figure 5

Double immunofluorescence histology analysis in murine colon. (A) Histochemical analysis of transduced murine colon in vivo. The X-Gal-positive cells were evident mainly on the luminal surface of the distal colon (magnification × 400). (B-F) Double immunofluorescence staining of tranduced murine colon examined under confocal microscopy. On Day 2 following rectal administration, β-Gal-positive cells in murine colon tissue expressed CD45, CD4, and cytokeratin, but not CD8 or CD11c. In these photomicrographs, β-Gal-positive cells are visualized by red fluorescence, CD phenotypes and cytokeratin are visualized by green fluorescence, and all images display fluorescence overlays: (B) β-Gal and CD45; (C) β-Gal and CD4; (D) β-Gal and CD8; (E) β-Gal and CD11c; and, (F) β-Gal and cytokeratin. Double-positive cells indicated by the arrowheads are yellow. (EP; epithelial cell, LP; lamina propria cell)

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