Figure 1
From: Novel application of low pH-dependent fluorescent dyes to examine colitis
Application of low pH-dependent fluorescent dyes to the mucosa of the normal colon. (A) HE stain and Alcian blue (pH 2.5) stain of the colonic mucosa obtained from a normal BALB/c mouse. Bar, 200 μm. (B) Stereomicroscopic observation of the colonic mucosa obtained from the normal BALB/c mouse. Bar, 200 μm. (C) Fluorescence stereomicroscopic observation of the colonic mucosa in PBS only. (D) Fluorescence stereomicroscopic observation of the colonic mucosa in PBS containing 1 μg/ml NBD-PZ after incubation at 37°C for three minutes. A magnified image of the indicated area is shown in the right panel. We observed five samples and obtained similar findings. (E) Stereomicroscopic and (F) fluorescence stereomicroscopic observation of the colonic mucosa in PBS containing 1 μg/ml LysoSensor Green DND-189 after incubation at 37°C for 20 minutes.