Increased phosphorylation of myosin light chain in intestinal epithelium in IO rats was inhibited by treatment with ML-7. (A) Western blots showing that IO augmented the mucosal levels of phosphorylated MLC (pMLC), which was attenuated by ML-7. n = 3-4/group. *p < 0.05 vs. respective sham rats. #p < 0.05 vs. IO rats. Representative images of pMLC staining in intestinal tissues of (B) sham, (C) IO, (D) sham+ML-7, and (E) IO+ML-7 rats. The staining of pMLC (green color) was localized to the villous surface (arrows) in IO rats, whereas no staining was seen in sham controls. The cell nuclei were shown in blue color. Addition of ML-7 prevented the IO-induced phosphorylation of epithelial MLC. Images: 200× magnification.