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Table 1 Characteristic values relating to the expression of each gene considered by Real time PCR

From: Bax Inhibitor-1 down-regulation in the progression of chronic liver diseases

Gene Threshold Slope Efficiency E = (10 -1/slope )-1 R 2 Range (copies/μl)
β-actin (n.24) 0.078 -3.34 ± 0.03 98.37 ± 3.43 0.998 10 8 -10 2
Bax (n.15) 0.104 -3.51 ± 0.05 94.67 ± 1.99 0.998 10 8 -10 3
BI-1 (n.18) 0.144 -3.48 ± 0.03 93.48 ± 1.34 0.999 10 8 -10 2
  1. Threshold value, manually adjusted, was maintained for all the standard curve analyzed. Table report the mean ±SD of the slope and efficiency value relative to n. 24, 15 and 18 standard curves of β-actin, Bax and BI-1 respectivelly. Efficiency was calculated from the slope using the equation E = (10-1/slope) -1. The correlation coefficient (square values R2) and range of standard curves are also reported.