BI-1 protein expression in HCC and tissues surrounding HCC. (3A): Western blot analysis of BI-1 protein expression in cirrhotic liver tissues surrounding hepatocellular carcinoma (PHCC) and in HCC tissues obtained from the same patient. Proteins were loaded onto 15% SDS-polyacrylamide gels and transferred to nitrocellulose membranes. Blots were incubated with anti-BI-1 and anti β-actin antibodies, followed by detection using an ECL method. (3B): Results of the densitometric analysis of BI-1. Data are expressed as the optical density ratio of BI-1 to β-actin. Values are reported as mean values ± SD.