Figure 5
Detection of ANEA of IgG and IgM class by IIF. Commercially available slides with Hep2 cells (Hep2 cells), cultured HeLa cells (HeLa cells) and purified nuclei from rat liver (R.L. nuclei) were incubated with various PBC sera and secondary anti-human FITC-labeled IgG and IgM antibodies, as described in Materials and Methods.
