Impaired fls485 expression in celiac disease. fls485 expression is impaired in human small intestinal mucosa affected with celiac disease classified Marsh IIIa-c when compared with normal mucosal specimens (controls). (A) fls485 mRNA expression in small intestinal mucosa detected with primer pair I. (B) fls485 mRNA expression detected with primer pair II in the identical probes as shown in (A). (C) Anti-fls485 immunohistochemistry (clone #7/2) of intestinal mucosa classified Marsh IIIb (original magnification approx. × 200). (D) Serial tissue section of (C) incubated with normal serum as negative control (original magnification approx. × 200). (E) Morphological aspects of CaCo2 cells cultured with low or high cellular density (original magnification approx. × 200). (F) fls485 expression normalized to GAPDH is increased in high density CaCo2 cell cultures (primer pair I, p < 0.05).