Characterization of fls485 expression in intestinal mucosa. fls485 expression in human intestinal mucosa with mRNA in situ hybridization (A and B), Western blot analysis (C and D), or immunohistochemistry (E and F). (A) Distribution of fls485 mRNA in human small intestinal mucosa (antisense riboprobe). The arrows mark strong mRNA accumulation in putative chromaffin cells (original magnification approx. × 200). (B) Serial section of (A) incubated with fls485 sense riboprobe (original magnification approx. × 200). (C) Blotted protein lysates from human small and large intestinal mucosa as well as Rko and Lovo cells after incubation with clone #7. (D) Blotted lysates as shown in (C) probed with clone #10. (E) fls485 protein is found with an increasing gradient along the CVA (clone #10) (original magnification approx. × 200). Chromaffin cells are marked by arrows. Note some interstitial cells are stained. (F) Strong fls485 immunostaining is found in a chromaffin cell (arrow), whereas Paneth cells (arrowheads) are only slightly or not stained (clone #10) (original magnification approx. × 400).