Co-localization of hmAchR M3-specific antibodies with patient-derived PBC-specific antibodies. Confocal laser scanning microscopy detecting co-localization of a hmAchR M3-specific antibody with patient-derived PBC-specific antibodies. HmAchR M3-positive HT-29 human colon carcinoma cells were fixed and stained with a hmAchR M3-specific polyclonal antibody (poAb) (lanes A+B, column 2) or the serum IgG fractions of a representative PBC patient (lane A, column 3) or a healthy control (lane B, column 3). As a positive control for cell surface receptor staining, also a Na+/K+-ATPase-specific mAb (lane C, column 3) was used. Magnification: 630×. Provided are the transmission (column 1), fluorescence data (columns 2+3) as well as the overlay (column 4) of the different photographs. The white arrows show the co-localization of both antibodies as indicated by the yellow color.