Lubiprostone ameliorates the cystic fibrosis mouse intestinal phenotype

BMC Gastroenterology

Table 2 Genes downregulated in the control CF intestine as compared to control WT, and upregulated by lubiprostone treatment of CF mice.

CF	WT+L	CF+L	Symbol	Name	Function/Process
0.01	0.4	0.11	Slc5a12	solute carrier family 5 (sodium/glucose cotransporter), member 12	Na-monocarboxylate transporter
0.05	0.4	0.33	Cyp4a10	cytochrome P450, family 4, subfamily a, polypeptide 10	20-HETE synthesis
0.08	0.9	0.46	Pck1	phosphoenolpyruvate carboxykinase 1, cytosolic	Glucose metabolism
0.12	0.6	1.1	Pdk4	pyruvate dehydrogenase kinase, isoenzyme 4	Glucose metabolism
0.15	1.3	0.53	Fbp1	fructose bisphosphatase 1	Glucose metabolism
0.21	1.2	2.14	Hmgcs2	3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2	Ketogenesis
0.21	1.1	1.74	Acot2	acyl-CoA thioesterase 2	Fatty acid metabolism
0.25	1.1	0.93	Slc13a2	solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2	Na-dicarboxylate transporter
0.25	1.1	1.33	Sgk1	serum/glucocorticoid regulated kinase 1	Regulation of electrolyte transport
0.33	1.6	1.32	Akr1b7	aldo-keto reductase family 1, member B7	PGF₂a synthesis
0.33	1.1	2.15	Angptl4	angiopoietin-like 4	Lipid metabolism

Genes whose expression level in the CF control intestine were ≥ 3-fold less than WT control and were upregulated ≥ 3-fold by lubiprostone in CF mice. Expression values are data from the GCOS analysis relative to WT control levels. Gene names are according to the Mouse Genome Informatics website http://www.informatics.jax.org/. +L = lubiprostone treated. Equal amounts of total RNA were pooled from 7-10 mice in each group and were used to interrogate the Affymetrix Mouse 430 2.0 GeneChip (n = 1 per group). The full microarray dataset is deposited in NCBI's Gene Expression Omnibus and is accessible through GEO Series accession number GSE18327 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE18327.

ISSN: 1471-230X