Lubiprostone ameliorates the cystic fibrosis mouse intestinal phenotype

BMC Gastroenterology

Table 1 Genes upregulated in the control CF intestine as compared to control WT, and downregulated by lubiprostone treatment of CF mice.

CF	WT+L	CF+L	Symbol	Name	Function/Process
1176	1.52	365	Retnlb	resistin like beta	Innate immunity
112	3.11	4.5	Chi3l4	chitinase 3-like 4	Innate immunity
51	0.52	8.7	Pla2g4c	phospholipase A2, group IVC (cytosolic, calcium- independent)	Inflammation, eicosanoid metabolism
42	0.76	3.4	Mcpt1	mast cell protease 1	Innate immunity, mast cell
23	1.32	2.8	Spna1	spectrin alpha 1	Erythrocyte cytoskeleton
18	0.23	1.3	Gsdmc2	gasdermin C2	Growth, differentiation, apoptosis
16	0.76	1.4	Mcpt2	mast cell protease 2	Innate immunity, mast cell marker
14	1.15	2.1	Mcpt9	mast cell protease 9	Innate immunity, mast cell
12	1.32	3.7	B3galt5	UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5	Sialyl Lewis-a synthesis
11	4.92	2.1	Ube2l6	ubiquitin-conjugating enzyme E2L 6	Innate immunity, ISG15- ubiquitination
8.6	1.00	1.2	Capn13	calpain 13	Growth, differentiation, apoptosis
8.0	0.23	1.4	Gsdmc4	gasdermin C4	Growth, differentiation, apoptosis
7.0	0.27	1.3	Gsdmc	gasdermin C	Growth, differentiation, apoptosis
4.9	0.44	1.4	Cpa3	carboxypeptidase A3, mast cell	Innate immunity, mast cell marker
4.0	0.76	0.2	Fcer1a	Fc receptor, IgE, high affinity I, alpha polypeptide	Innate immunity, mast cell marker
3.7	1.15	1.4	Egln3	EGL nine homolog 3 (C. elegans)	Prolyl hydroxylation of HIF
3.5	1.62	1	(none)	single chain antibody ScFv	Immunoglobulin heavy chain, immunity
3.2	0.56	0.5	Scd2	stearoyl-Coenzyme A desaturase 2	Unsaturated fatty acid synthesis

Genes whose expression level in the CF control intestine were ≥ 3-fold greater than WT control and were downregulated ≥ 3-fold by lubiprostone in CF mice. Expression values are data from the GCOS analysis relative to WT control levels. When there were multiple probe sets for a particular gene the values were averaged. Gene names are according to the Mouse Genome Informatics website http://www.informatics.jax.org/. +L = lubiprostone treated. Equal amounts of total RNA were pooled from 7-10 mice in each group and were used to interrogate the Affymetrix Mouse 430 2.0 GeneChip (n = 1 per group). The full microarray dataset is deposited in NCBI's Gene Expression Omnibus and is accessible through GEO Series accession number GSE18327 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE18327.

ISSN: 1471-230X